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      多糖衍生物鍵合型手性色譜柱

      首頁 色譜柱與填料 多糖衍生物鍵合型手性色譜柱
      概述
      耐溶劑型手性色譜柱是通過化學鍵合型結(jié)合的方式將多糖衍生物固定在硅膠上。
      現(xiàn)在已有12款:CHIRALPAK? IA/IB N※/IC/ID/IE/IF/IG/IH/IJ/IK/IM/IN(簡稱 iCHIRAL Series), iCHIRAL Series可拆分的手性化合物廣泛。
      ※CHIRALPAK? IB N-5/IB N-3與CHIRALPAK? IB/IB-3(填料粒徑:5μm/3μm)的手性填料均為纖維素三(3,5-二甲基苯基氨基甲酸酯)共價鍵合在硅膠表面,IB N-5/IB N-3在保持IB/IB-3分離傾向的同時,大幅改善了分離度,不僅在分析中提高了分離性能,應用于分離純化時也可提升制備效率。
      iCHIRAL Series的特性
      溶劑使用廣泛
      可使用各種互溶的溶劑作為流動相和溶解樣品的溶劑,溶劑使用的多樣性會帶來新的選擇能力。
      正相:n-Hexane, IPA, EtOH
      特殊流動相:Ethyl acetate,CH?Cl, THF, MTBE
      反相:H?O, IPA, MeOH
      高分離能力
      新型手性填料(CHIRALPAK? IC/ID/IE/IF/IG/IK)的加入,大大提高了分離能力。而且iCHIRAL Series系列所有型號手性柱都有填料粒徑為3μm的規(guī)格。部分型號推出了1.6μm填料粒徑的規(guī)格,適用于UHPLC,進一步提高了分離能力。
      良好的耐受性
      iCHIRAL Series手性柱是通過化學鍵合的方式將多糖衍生物固定在硅膠上,對于乙醇、乙醚、乙酸乙酯以及鹵代溶劑具有良好的耐受力,能夠?qū)⒎磻汉唵芜^濾后進到手性柱中,節(jié)省了分析前處理的時間。
      可再生修復
      在使用一段時間以后,手性柱的分離能力可能會由于正常損耗而有所下降。iCHIRAL Serise可使用特定的溶劑來進行手性柱再生,以恢復手性柱的分離能力。再生方法請參考使用說明書。
      使用壽命長
      因填料與硅膠之間結(jié)合方式牢固,可耐受多種溶劑。在復雜的溶劑中更穩(wěn)定,不用擔心手性柱因溶劑使用不當而使涂敷的填料被溶解,造成手性柱損壞。
      正/反相模式均適用
      可通過合適的溶劑置換,實現(xiàn)同支手性柱既可用于正相模式也可用于反相模式。提供內(nèi)徑為2.1mm規(guī)格的微粒柱,使用反相流動相體系時,可在LC-MS中使用。
      流動相
      iCHIRAL Series系列可使用各類溶劑作為流動相或者溶解樣品的溶劑(請注意溶劑之間的互溶性),舉例如下:
      • 正己烷 (n-Hexane)

      • 二甲基亞砜 (DMSO)

      • 異丙醇 (IPA)

      • 氯仿 (Trichloromethane)

      • 甲基叔丁基醚 (MTBE)

      • 乙腈 (Acetonitrile)

      • 二氯甲烷 (Dichloromethane)

      • 甲苯 (Methylbenzene)

      • 四氫呋喃 (THF)

      • 1,4-二氧六環(huán)(1,4-dioxane)

      • 乙酸乙酯 (Acetic ether)

      • 水 (H2O)

      應用實例
      iCHIRAL Series系列可以選擇各種溶劑作為流動相,擴大了色譜分析和制備條件的范圍內(nèi),可在實驗過程中選擇適合的流動相條件。

      由于可以使用各種溶劑,因此可以將“反應混合物”直接注入耐溶劑手性柱中,從而節(jié)省分析前處理的麻煩和時間。
      相關(guān)文獻
      ● Profiling of branched chain and straight chain saturated fatty acids by ultra-high performance liquid chromatography-mass spectrometry, X. Fu, N. Hafza, F. G?tz, M. L?mmerhofer, J. Chromatogr. A, 1703, issue #16, (2023)
      ● 
      The Chiral Separation of the (+) and (-) Enantiomers of Cannabidiol, W. Umstead, Cannabis Science and Technology, 5, issue #5, (2022), 30-34
      ● 
      The separation of several organophosphate pesticides on immobilized polysaccharide chiral stationary phases, W. Champion Jr, W. Watts Jr, W. Umstead, Chirality, 34, issue #8, (2022), 1078-1093
      ● 
      ON/OFF receptor-like enantioseparation of planar chiral 1,2-ferrocenes on an amylose-based chiral stationary phase: The role played by 2-propanol, C. Cantatore, M. Korb, H. Lang, R. Cirilli, Analytica Chimica Acta, 1211, (2022)
      ● 
      Characteristic and complementary chiral recognition ability of four recently developed immobilized chiral stationary phases based on amylose and cellulose phenyl carbamates and benzoates, T. Onishi, T. Ueda, K. Yoshida, K. Uosaki, H. Ando, R. Hamasaki, A. Ohnishi, Chiralty, (2022), 1-16
      ● 
      Investigation of retention behavior of natural cannabinoids on differently substituted polysaccharide-based chiral stationary phases under reversed-phase liquid chromatographic conditions, C. De Luca, A. Buratti, W. Umstead, P. Franco, A. Cavazzini, S. Felletti, M. Catani, J. Chromatogr. A, 1672, (2022)
      ● 
      Single-run chemo- and enantio-selective high-performance liquid chromatography separation of tramadol and its principal metabolite, O-desmethyltramadol, using a chlorinated immobilized amylose-based chiral stationary phase under multimodal elution conditions, C. Cantatore, G. La Regina, R. Ferretti, R. Silvestri, R. Cirilli, Sep Sci Plus, (2022), 1-6
      ● 
      Towards enantioselective ultrahigh performance liquid chromatography–mass spectrometry-based metabolomics of branched-chain fatty acids and anteiso-fatty acids under reversed-phase conditions using sub-2-μm amylose- and cellulose-derived chiral stationary phases, C. Geibel, L. Zhang, K. Serafimov, H. Gross, M. L?mmerhofer, Chiralty, (2022), 1-14
      ● 
      DoE Optimization Empowers the Automated Preparation of Enantiomerically Pure [18F]Talazoparib and its In Vivo Evaluation as a PARP Radiotracer, G. D. Bowden, S. Stotz, J. Kinzler, C. Geibel, M. L?mmerhofer, B. J. Pichler, A. Maurer, J. Med. Chem., 64, issue #21, (2021), 15690–15701
      ● 
      The Separation of Cannabinoids on Sub-2 μm Immobilized Polysaccharide Chiral Stationary Phases, T. Onishi, W. J. Umstead, Pharmaceuticals, 14, issue #12, (2021)
      ● 
      The Separation of Several Minor Cannabinoids via Chiral HPLC, W. J. Umstead, Cannabis Science and Technology, 4, issue #6, (2021), 44-51
      ● 
      Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometry, F. Mueller, G.L. Losacco, R. Nicoli, D. Guillarme, A. Thomas, E. Grata, J. Chromatogr B., (2021)
      ● 
      Solvent versatility of immobilized 3,5-dimethylphenylcarbamate of amylose in enantiomeric separations by HPLC, T. Zhang, C. Kientzy, P. Franco, A. Ohnishi, Y. Kagamihara, H. Kurosawa, J. Chromatogr. A, 1075 (2005) 65-75
      ● Cellulose 3,5-dimethylphenylcarbamate immobilized on silica: A new chiral stationary phase for the analysis of enantiomers, T. Zhang, D. Nguyen, P. Franco, T. Murakami, A. Ohnishi, H. Kurosawa, Anal. Chim. Acta, 557 (2006) 221-228
      ● Cellulose tris(3,5-dichlorophenylcarbamate) immobilised on silica: A novel chiral stationary phase for resolution of enantiomers, T. Zhang, D. Nguyen, P. Franco, Y. Isobe, T. Michishita, T. Murakami, J. Pharm. Biochem. Anal., 46 (2008) 882-891
      ● Common approaches for efficient method development with immobilised polysaccharide-derived chiral stationary phases, P. Franco, T. Zhang, J. Chromatogr. B, 875 (2008) 48
      ● Enantiomer resolution screening strategy using multiple immobilised polysaccharide-based chiral stationary phases, T. Zhang, D. Nguyen, P. Franco, J. Chromatogr. A, 1191 (2008) 214-222
      ● Complementary enantiorecognition patterns and specific method optimization aspects on immobilized polysaccharide-derived chiral stationary phases, T. Zhang, P. Franco, D. Nguyen. R. Hamasaki, S. Miyamoto, A. Ohnishi. T. Murakamo, J. Chromatogr. A, 1269 (2012) 178–188

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